Potential Mechanisms of Yiqi Jiedu Huayu Decoction in the Treatment of Diabetic Microvascular Complications Based on Network Analysis, Molecular Docking, and Experimental Validation

Background. Diabetic microvascular complications are the main causes of organ dysfunction and even death in diabetic patients. Our previous studies confirmed beneficial effects Yiqi Jiedu Huayu Decoction (YJHD) on cardiomyopathy nephropathy. It is not clear whether YJHD can treat multiple including retinopathy, cardiomyopathy, nephropathy through some common mechanisms. Methods. TCMSP, SymMap, STITCH, Swiss Target Prediction, SEA databases were used to collect analyze components targets YJHD. GeneCards, DrugBank, DisGeNET, OMIM, GEO obtain target genes for The GO KEGG enrichment analyses performed DAVID STRING platforms. Molecular docking was evaluate binding sites affinities compounds proteins. Animal experiments designed validate network pharmacology results. Results. Through pharmacological analysis, oxidative stress, inflammatory response, apoptosis identified as key pathological phenotypes treatment with In addition, JNK, p38, ERK1/2 predicted regulating abovementioned phenotypes. results animal showed that could ameliorate retinal changes diabetes rats. inhibit stress inflammation heart kidney strong between ERK1/2. Berlambine may play a role process considered promising regulator MAPK protein family. regulatory demonstrated experiments. Conclusions. therapeutic by apoptosis. regulation phosphorylation be its effect.